Regulatory
Part:BBa_K2365040
Designed by: yuanqi jia Group: iGEM17_NAU-CHINA (2017-10-14)
PGK1 promoter
PGK1 Yeast promoter Constitutive expression
Phosphoglycerate kinase, phosphoglycerate kinase promoter, transcription start key enzyme of glycolysis, and each species to enzyme must survive, the deficiency of the enzyme can cause organism metabolism disorder, as a strong promoter in gene engineering
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.
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Categories
Parameters
None |