Translational_Unit

Part:BBa_I737020:Design

Designed by: Vladislav Lavrovsky   Group: iGEM07_Calgary   (2007-10-26)
Revision as of 21:30, 26 October 2007 by Vlad (Talk | contribs) (→‎Design Notes)

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PoPS->Agarase (Digests agar)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1861
    Illegal NheI site found at 106
    Illegal NheI site found at 901
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1861
    Illegal XhoI site found at 1964
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
    Illegal AgeI site found at 368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1319
    Illegal SapI.rc site found at 1717
    Illegal SapI.rc site found at 1795


Design Notes

There is a EcoRI and a PstI site in the sequence which must be mutated at some point. The UofC team did not have time to do the appropriate site-directed mutagenesis. Construction is possible using XbaI and SpeI. The Calgary team has sucessfully attempted non-directional cloning of this gene into another BioBrick (J13002), part (I737021) which will be available as soon as possible.

Source

Cloned out of Pseudoalteromonas atlantica T6C from the ATCC.

References