Part:BBa_K1061011

EF-1alpha promoter

Ef-1alpha promoter is a strong constitutive promoter that drive the expression of elongation factor -1α.It has been widely used in constructing mammalian cell-based circuit, especially useful for construction of long-term circuit in some cells including iPS cells and Jurkat cells in which the strong promoter CMV may be silenced.[1]

Further Characterization - Team Technion 2017

We believe this is not the full EF-1alpha promoter, but rather the core promoter of EF-1alpha (Approx. 300bp) attached to the R segment and part of the U5 sequence (R-U5’) of the Human T-Cell Leukemia Virus (HTLV) Type 1 Long Terminal Repeat.

The full EF-1alpha promoter is approx. 1200bp, and contains two forbidden restriction sites (by iGEM Biobrick standards).

Team Technion 2017 created a mutated version of the full EF-1alpha promoter BBa_K2520023. This promoter is fully functional and lacks the forbidden restriction sites.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 344
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 440
  
• 1000
  COMPATIBLE WITH RFC[1000]

References:

[1] R.V. Gopalkrishnan, K.A. Christiansen et al, 1999,Use of the human EF-1α promoter for expression can significantly increase success in establishing stable cell lines with consistent expression: A study using the tetracycline-inducible system in human cancer cells, nucleic acids research,27:4775-4782