Coding

Part:BBa_K2271008

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
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Pex34-mTurquoise with galactose inducible promoter

This part contains the coding sequence for the peroxin Pex34p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein mTurquoise fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.

Usage and Biology

Pex34p is a peroxisomal integral membrane protein that can act both, independently and in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
    Illegal BamHI site found at 972
    Illegal XhoI site found at 1695
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None