Part:BBa_K530008:Experience

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Team Kyoto 2017

S. cerevisiae expressing GFP from this TDH 3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )

picture Figure1 : Observation of S. cerevisiae expressing EGFP by this TDH3 promoter with a fluorescence microscope.

B. xylophilus was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of B. xylophilus was filled with EGFP (photo or link). (fig 2 )

picture Figure2 : B. xylophilus full of EGFP in its esophagus.

This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in S. cerevisiae, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that BBa_K530008 and BBa_K1875003 work effectively as a new usage that has not been attempted so far, greatly extending the function of each part.

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