Part:BBa_K2365051

TPI1 promotor

TPI1 Yeast promoter;Triose-phosphateisomerase, triosephosphate isomerase promoter. Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence .it plays an important role in glycolysis, for energy generation is essential for effective, has been found in almost all organisms, including mammals, insects, fungi, plants and most bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 47
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 119
  

Nature promoter from Saccharomyces cerevisiae genome amplification obtained constitutive promoter, the original gene are associated with the metabolism of yeast. The other life from the genome of Cyc1 was amplified by overlap extension terminator promoter-cyc1t fusion fragments between elements of the same source on the arm pairing reserved standard restriction sites, even on the expression vector prs423 GFP (S65T), inserted into yeast sey6210 test 440-550nm fluorescence intensity.