Coding

Part:BBa_K2365037

Designed by: Wu Yaxuan   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 11:42, 31 October 2017 by Vickrystal (Talk | contribs)


Trichothecene 3-O-acetyltransferase(AYT1)，an indispensable enzyme for the biosynthesis of trichothecenes, to catalyzes the transfer of an acetyl group from acetyl coenzyme A to the C3 hydroxyl moiety which will result in a switch from DON to 3ADON, so the toxicity will be reduced by 100-fold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 306
    Illegal BglII site found at 318
    Illegal XhoI site found at 884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

PCR and SDS-PAGE

Showing the size of the target gene.

Don degrading enzyme 1.jpg DON degrading enzyme SDS.jpg


Uv don.png

[edit]
Categories
Parameters
None