Part:BBa_K2259081

Base vector 2.0 - a backbone for SynORI multi-plasmid framework

This is the updated version of iGEM base vector Part:BBa_I51020 that has been withdrawn from database due to encoding a ccdB toxin. Compared to BBa_I51020 the ccdB toxin was switched out to blue and white selection for picking colonies with replaced origin of replication. Part:BBa_I51020 page can still be use as a reliable source for this part's design and function.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 968
  Illegal suffix found at 2107
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 968
  Illegal NheI site found at 745
  Illegal NheI site found at 2283
  Illegal SpeI site found at 2108
  Illegal PstI site found at 2122
  Illegal NotI site found at 974
  Illegal NotI site found at 2115
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 968
  Illegal BglII site found at 1451
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 968
  Illegal suffix found at 2108
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 968
  Plasmid lacks a suffix.
  Illegal XbaI site found at 983
  Illegal SpeI site found at 2108
  Illegal PstI site found at 2122
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

About SynORI

SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!

Base vector 2.0 in SynORI framework

Engineering an improved, functional base vector 2.0 is crucial for SynORI framework.

Building a modular a synthetic origin of replication is easiest when working in biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site.

One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transfered to another plasmid location and biobricks are then free to use for any task.

See [http://2017.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania 2017 team wiki] for more insight about our synthetic origin of replication (SynORI).