Composite

Part:BBa_K2404016

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 09:30, 31 October 2017 by Gparry75 (Talk | contribs)


A TSH antagonist with His-Tags, controlled by the LexA promoter

This construct contains the LexA promotor, the [TSHH coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.


Sequence and Features 1-6: BsmBI:
7-348: LexA:
349-2005: LUC+:
2006-2272: NosT:
2282-2287: BsmBI:


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1143
    Illegal XhoI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]



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