Composite

Part:BBa_K2371009:Design

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-23)
Revision as of 03:15, 1 November 2017 by Mat2017 (Talk | contribs)

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sgRNA generator for EML4-ALK variant B 126


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The EML4-ALK fused gene has two variants (A and B).

We obtained the sequence of EML4-ALK from NCBI.

https://www.ncbi.nlm.nih.gov/nuccore/AB374361.1 EML4-ALK variant 3 a

https://www.ncbi.nlm.nih.gov/nuccore/AB374362.1 EML4-ALK variant 3 b

The part was synthesized by SOEPCR designed by DNAworks.

References

1. Wong DW, et al. The EML4-ALK fusion gene is involved in various histologic types of lung cancers from nonsmokers with wild-type EGFR and KRAS. Cancer. 2009 Apr 15;115(8):1723-33.

2. DNAWorks: https://hpcwebapps.cit.nih.gov/dnaworks/