Part:BBa_K2440016

miR-511 target sequence

It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.

Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.¹ It has been reported that hsa-miR-511-5p can act as an intracellular mediator of glucocorticoids and TGF-β for the induction of endotoxin tolerance in human monocytes.²

Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL patients.³

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 4
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 4
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 4
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 4
  
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.