Coding
TSHHantag
Part:BBa_K2404001:Design
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-09-22)
A Thyroid Stimulating Hormone antagonist with His-Tags
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 540
Illegal PstI site found at 582 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 540
Illegal PstI site found at 582 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 795
Illegal XhoI site found at 607 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 540
Illegal PstI site found at 582 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 540
Illegal PstI site found at 582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 827
Design Notes
We generated this part through the following steps:
- We ordered the following TSH gBlock from IDT
- This was introduced into pSB1C3 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning
Source
This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags. It was ordered as a g-block.