Coding

Part:BBa_K2365048

Designed by: HangYu Duan   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 10:50, 31 October 2017 by 216 (Talk | contribs) (→‎300px|center)


Bax mutant form1

Coding sequence of Bax mutant form1 protein which releases cytochrome C and leads to apoptosis in the most broadly used eukaryotic chassis. This year we choose Saccharomyces cerevisiae as our chassis fungal, so we use this gene as the key gene in our biosafety device. It worked well when we detected it's function.We submitted as a improvement of already exist hbax biobrick. The link of original biobrick is here:https://parts.igem.org/Part:BBa_K364202

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Bax.png

Figure1: Picture of gel electrophoresis (PCR validated products of Bax mutant form1 ; 579bp) Function： We use Bax protein to kill our engineered yeast when the fungal has finished its mission.

图片3.png

图片4.png

图片5.png

图片6.png

图片7.png

图片8.png
图片9.png
图片10.png
[edit]
Categories
Parameters
None