Regulatory

Part:BBa_K2348000:Design

Designed by: Andreas Berner   Group: iGEM17_NAWI_Graz   (2017-10-19)
Revision as of 15:36, 30 October 2017 by Andi332 (Talk | contribs) (→‎References)


alx - alkaline regulated riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 312


Design Notes

The alx regulatory part contains the constitutiv alx promoter and the riboswitch itself


Source

Colony PCR from E. coli BL21

References

1Bingham, R. J., Hall, K. S. & Slonczewski, J. L. Alkaline induction of a novel gene locus, alx, in Escherichia coli. Journal of bacteriology 172, 2184–2186 (1990).

2Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A., Westhof, E. & Altuvia, S. A pH-responsive riboregulator. Genes & development 23, 2650–2662 (2009).