Tag

Part:BBa_J54025:Design

Designed by: Yusaku Nakashima   Group: iGEM06_Tokyo_Alliance   (2006-10-16)
Revision as of 17:08, 25 October 2007 by Iwasaki (Talk | contribs) (→‎Design Notes)

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SuffixI_BglII,MluI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As Suffix of Tokyo Standard.


Tokyo Standard consists of the chain of restriction sites : SalI-BamHI-part-BglII-MluI


BamHI sequence is "G^GATCC", and BglII sequence is "A^GATCT".

They have the sequence "GATC" in common, so you can assemble parts just like Biobrick assembly.

Source

DNA synthesis

References