Composite

Part:BBa_K2474000:Design

Designed by: J. Baggman, J. Bergqvist, H. Karlsson, L. Karlsson, J. Larsson, M. Lindberg, M. Nilsson, M. Peterson, O. Reinhed Gustafsson, S. Stridh Karppinen & J. Ybrahim   Group: iGEM17_Linkoping_Sweden   (2017-10-27)
Revision as of 08:57, 1 November 2017 by Moani870 (Talk | contribs) (→‎Design Notes)


Amyloid-B mNeonGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1267
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2190
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We designed this plasmids with amyloid-beta bound to the fluorescent protein mNeonGreen with C-terminus. For this plasmid we used an arabinose promotor called AraC which is induced by addition of arabinos. To connect the fusion proteins we had to create a GS-linker between Amyloid-Beta and mNeonGreen to make it more motile.

Source

mNeonGreen and Amyloid-Beta was synthetically made by the company IDT. pSB1C3 came form iGEM and pBAD is a BioBrick (BBa_10500).

References