Coding

Part:BBa_K2333011:Design

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-10-27)
Revision as of 13:35, 30 October 2017 by Cemonette (Talk | contribs) (→‎References)

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mf-Lon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1834
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 2124
    Illegal AgeI site found at 2149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This specific sequence was codon-optimized for E. coli using the website www.jcat.de

Source

This sequence was adapted from Gur, Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease". Synthesized by IDT.

References

Cameron DE, Collins JJ. (2014) Tunable protein degradation in bacteria. Nature Biotechnology, 32(12):1276–1281.

Gur E, Sauer RT. (2008) Evolution of the ssra degradation tag in mycoplasma: specificity switch to a different protease. Proceedings of the National Academy of Sciences,105(42):16113– 16118.