Regulatory
Plac(rev)

Part:BBa_I742124

Designed by: Caroline Dahl   Group: iGEM07_Edinburgh   (2007-10-22)
Revision as of 16:53, 25 October 2007 by Cfrench (Talk | contribs)

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Reverse complement Lac promoter

This is an reverse complement lac promoter from Escherichia coli. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory possibility that this gives.

The upstream sequence is non-functional and simply there to facilitate gel analysis or to physically enable the part to be recombineered (see the Edinburgh 2007 project where it is flanked by dif-sites).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/reverse
//chassis/prokaryote/ecoli
//promoter
//regulation/multiple
Parameters
negative_regulators1
positive_regulators