Composite

Part:BBa_K2247001

Designed by: Jianing Han   Group: iGEM17_Tsinghua   (2017-10-23)
Revision as of 10:27, 30 October 2017 by GBY15 (Talk | contribs)


pGalS-GFP

This part encodes a GFP gene (BBa_K895006) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see BBa_K2247000).


Usage and Biology

See BBa_K2247000.

This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1130


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
chassisS. cerevisiae
functionGal4-dependent inducible expression
positive_regulatorsyeast Gal4 protein