Terminator

Part:BBa_K2440006

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 07:08, 30 October 2017 by Jhyzxqj (Talk | contribs) (→‎Usage and Biology)


Synthetic PA

The Poly A, also called Polyadenylation, is the addition of a poly adenine bases tail to a messenger RNA. It is important for the nuclear export, translation, and stability of mRNA. 1


Usage and Biology

The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. 2-3

At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None