T7

Part:BBa_K2382003

Designed by: TING-YU LIN   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 03:41, 30 October 2017 by Herlohuang (Talk | contribs) (→‎Usage and Biology)

T7 promoter & Lac operator and RBS from PET-29a


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.

We tried to improve this promoter sequence at BBa_K525998 by adding an lac operator and making it RFC 10 compatible.

Characterization of the T7 promoter & Lac operator and RBS from PET-29a

References

pET-29a(+) 5.4kb - GenScript https://www.genscript.com/gsfiles/vector-map/bacteria/pET-29a.pdf

[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
Parameters
None