Coding

Part:BBa_K2271066

Designed by: Jan Maika and Philipp Rink   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 00:35, 30 October 2017 by Philipp R (Talk | contribs) (→‎Usage and Biology)


mRuby-ePTS1

Usage and Biology

This Part is a composite Part containing the fluorescent protein mRuby targeted to the peroxisome with an enhanced PTS1 (Dueber et al.). The part is designed as a peroxisomal lumen marker for S. cerevisiea. using the TDH3 promoter and the HHF1 terminator. For fluorometric and microscopic applications the an optimal excitation of 559 nm and emission of 600 nm is discriped.

Experimental Design and Results

For the validation the S. cerevisiae Strain BY4247 was tranformed with this part. The cells were fixated and microscope with an xxx microscope. A typical peroxisomal localisation could be validated (Figure 1).

Figure 1 mRuby fused with PTS1 localised peroxisomal typical in the cells




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
    Illegal BamHI site found at 1577
    Illegal XhoI site found at 1613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



[edit]
Categories
Parameters
None