Coding

Part:BBa_K2271066

Designed by: Jan Maika and Philipp Rink   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 00:12, 30 October 2017 by Philipp R (Talk | contribs) (→‎Experimental Design and Results)


mRuby-ePTS1

Usage and Biology

The part contains a mRuby fluorezenz protein with an enhanced pts1 sequence, described by Dueber et. Al, with a TDH3 Promotor and a HHF1 Terminator. mRuby is a mutant oft he fluorezent protein gfp with excitation and emission wavelengths at 559 nm/600nm . With the C-terminal enhanced pts1 (peroxisomal targeting sequnce) the mRuby is imported into the peroxisome. The part can be used as a peroxisomal matrix marker in S. cerevisiea.

Experimental Design and Results

For the validation the S. cerevisiae Strain BY4247 was tranformed with this part. The cells were fixated and microscope with an xxx microscope. A typical peroxisomal localisation could be validated (Figure 1).

Figure 1 mRuby fused with PTS1 localised peroxisomal typical in the cells




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
    Illegal BamHI site found at 1577
    Illegal XhoI site found at 1613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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