RBS

Part:BBa_K2333008:Design

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-10-27)
Revision as of 20:28, 29 October 2017 by Cemonette (Talk | contribs) (→‎Source)


mScarlet-I no stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Stop codons have been removed for the purpose of combination with protein degradation tags. The sequence for this part has been codon-optimized for E. coli.

Source

mScarlet-I was originally designed by Bindels et al. 2016 "MScarlet: a bright monomeric red fluorescent protein for cellular imaging". This specific sequence was synthesized by IDT.

We use the website www.jcat.de to optimize the codon sequence for E. coli.

References