Device

Part:BBa_K2213001:Experience

Designed by: Jessica Burns   Group: iGEM17_Manchester   (2017-08-17)
Revision as of 20:23, 29 October 2017 by Adam hannaford (Talk | contribs) (Applications of BBa_K2213001)


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Manchester 2017 - BBa_K2213001

To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including: - concentration of Tetracyclin inducer (induces EutMN synthesis) - concentration of IPTG inducer (induces EutS synthesis) - Harvest time (time after induction) - Temperature - Growth Medium (LB and TB) / from this 22 flask investigation we were able to make surface plots to visulise our findings. /

      • 2 TEMPERATURE DoE SURFACE PLOTS***

/ Figure1:

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