Measurement
Part:BBa_K2333407
Designed by: Ethan M Jones Group: iGEM17_William_and_Mary (2017-06-29)
UNS J23100 sfGFP pdt A
This part is designed to facilitate easy measurement of the strength of protein degradation tag (pdt) A by measuring time to steady-state fluorescence values of sfGFP under the control of the strong constitutive promoter J23100. William and Mary iGEM 2017 used pdts as a method to control gene expression speed.This part was utilized to characterize the degradation properties of pdt A and confirm the fact that different pdts have different degradation strengths. See [http://2017.igem.org/Team:William_and_Mary/Results William and Mary's 2017 project] for more details This part is one of a series of easy sfGFP reporter pdt parts. Series range is from BBa_K2333401 to BBa_K2333406
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 106
[edit]
Categories
Parameters
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