Regulatory
Pu (second

Part:BBa_I723138:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)
Revision as of 16:39, 25 October 2007 by Scott.w.ramsay (Talk | contribs) (→‎Source)


Pu (secondary version)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 238
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[Christine/Maia]


Source

Cloned from the TOL plasmid from Pseudomonas putida MT2. This is the promoter that regulates the TOL catabolic plasmid upper pathway operon.

References

Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.

Harayama, S., Leppik, R.A., Rekik, M., Mermod, N., Lehrbach, P.R., Reineke, W., and Timmis, K.N. 1986. Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product. Journal of Bacteriology 167, 455-461.