Coding

Part:BBa_K2374003:Design

Designed by: Qian Zeng   Group: iGEM17_Tongji_China   (2017-10-20)
Revision as of 00:42, 30 October 2017 by Zjeng (Talk | contribs)


ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1157
    Illegal XhoI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 535
    Illegal BsaI site found at 1501
    Illegal BsaI.rc site found at 253
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 1168


Design Notes

site direct mutangenesis: Pst I (647) CTGCAG->CTGCTG

According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.

标题
               <img src="2017tongji_wiki_image_process_13.png" alt="1% gel electrophoresis of plasmid" style="width:100%">

                 pUAST-pleP-Gal80ts

Source

Drosophila melanogaster chromosome 3L [NT_037436.4] (NCBI)


References

JANICE A. FISCHER, et al. GAL4 activates transcription in Drosophila. Nature 332, 853 - 856 (1988)