Part:BBa_K2325101
Formamidase expression device
The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
Usage and Biology
Using the pGEX-2T vector, the FOR could be expressed in BL21(DE3).
Figure 1. Growth curves of E.coli containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls
To verify that our recombinant E. coli containing formamidase gene could work efficiently to utilize formamide as its nitrogen sources, we cultivated the recombinant one and the negative controls in a basal MOPS medium with formamide as the sole nitrogen sources. E. coli containing formamidase gene, reached maximum concentration after 48 hours in the basal MOPS medium in the presence of 200 mM formamide. While E.coli without formamidase gene basically did not grow.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 189
Illegal NgoMIV site found at 676
Illegal NgoMIV site found at 871
Illegal NgoMIV site found at 1018 - 1000COMPATIBLE WITH RFC[1000]
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