Generator

Part:BBa_K2486007

Designed by: Cau Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)
Revision as of 00:58, 2 November 2017 by Gabriellovate (Talk | contribs) (→‎Usage and Biology)

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lldR repressor generator

A part that constitutively generates the LldR repressor

Usage and Biology

This part consists of:

  1. A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
  2. An RBS based on Elowitz repressilator (BBa_B0034).
  3. The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
  4. A terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 874
    Illegal NheI site found at 897
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None