Reporter

Part:BBa_K2448001:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 11:13, 29 October 2017 by Brumake (Talk | contribs)


mEmerald


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically optimised for Escherichia coli K12 with the help of [http://www.jcat.de/ JCAT]. Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.

Source

DNA synthesis

References

[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.

[2] Addgene plasmid # 53976