![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
2*RE6/RE5
Part:BBa_K2446002:Design
Designed by: Yijie Pan Group: iGEM17_Fudan (2017-10-21)
2_ZF54-8/43-8 binding site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, whichis made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
Source
IDT (gBlocks)
References
Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012).