Coding

Part:BBa_K2325002

Designed by: Huixian Li   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-14)
Revision as of 02:36, 28 October 2017 by KahiuLau (Talk | contribs) (→‎Usage and Biology)

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Formamidase & Phosphite dehydrogenase fusion protein

It is a fusion protein of formamidase and phosphite dehydrogenase with a linker between them.

Usage and Biology

We cloned FOR and PTDH genes into the plasmid pGEX-2T, and got the recombinant plasmid pGEX-for-ptx where FOR and PTDH form a fusion protein.

Gel analysis

BBa K2325002--1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None