Part:BBa_K2290003

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG of 3' end. The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].

[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.