Composite

Part:BBa_I751011:Design

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)
Revision as of 16:52, 24 October 2007 by Iwasaki (Talk | contribs) (→‎Design Notes)

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pcI-lux-lac-mCherry on pBR Standard


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 5121
    Illegal PstI site found at 5135
    Illegal NotI site found at 2768
    Illegal NotI site found at 5128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 2890
    Illegal BglII site found at 3572
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 5121
    Illegal PstI site found at 5135
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920


Design Notes

1. Move BBa_S03816 in the downstream of pBR Biobrick Tokyo Standard plasmid.

2. Introduce pcI promoter (bought from oligo house) in the upstream of Tokyo Standard site of pBR standard plasmid.

Source

References