Regulatory
Part:BBa_K2448012:Design
Designed by: jeremy armetta Group: iGEM17_Evry_Paris-Saclay (2017-10-27)
pPsiA from Sinorhizobium fredii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 345
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pPsiA sequence from Sinorhizobium fredii NGR234 plasmid pNGR234b (GenBank CP000874.1), nt 1113914 to 1114314 with 2 modifications to remove a BsaI site and a PstI site
Source
DNA synthesised based on the pPsiA sequence from Sinorhizobium fredii NGR234 plasmid pNGR234b (GenBank CP000874.1), nt 1113914 to 1114314 with 2 modifications to remove a BsaI site and a PstI site