Coding

Part:BBa_K2271104:Design

Designed by: Jan Maika   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 12:12, 28 October 2017 by Manule94 (Talk | contribs) (→‎Design Notes)


PEX13 membrane anchor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The membrane domain PEX13 (amino acids 200-310) was used as a peroxisomal membrane anchor for fluorescence proteins. We therefore added a triple GGGGS linker to allow an undisturbed c-terminal fusion.

Source

UniProtKB - P80667 (PEX13_YEAST)

References