Reporter

Part:BBa_K2305003

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-10-19)
Revision as of 18:06, 27 October 2017 by Buqi (Talk | contribs) (→‎Agarose gel electrophoresis)


pbad promoter with GFP and lacI

This is a pbad promoter expressing LacI and GFP

Agarose gel electrophoresis

We calculated the new part’s base number to be 2195bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1303
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1982


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