Coding

Part:BBa_K2271116:Design

Designed by: Fiona Edenhofer   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 14:23, 29 October 2017 by PhilippMueller (Talk | contribs)

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ADH PTS1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 2047
    Illegal XhoI site found at 2083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2433
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ADH we used for our project is from Pichia pastoris. It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. Usually an additionally cofactor regeneration is not necessary, as BM3 degrades NADH+H+ into NAD+ and ADH regenerates it. But because of the improved (+)-nootkatone producing function of BM3, another cofactor regenerating enzyme is necessary. In recent studies it was shown that Glucose Dehydrogenases (GDH) are appropriate enzymes for this attempt.

This biobrick additionally holds a PTS1 sequence for peroxisomal import.


Source

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References