Part:BBa_K2486101

Trypanolytic nanobody Nb_An46 (no stop codon)

[theoretical part] [not assembled, not synthesized, not sent, not characterized]

Nanobody targeting Trypanosoma bursei, the cause of african sleeping sickness, transmitted by the Tse-Tse fly.

GenBank acession: AY263491 https://www.ncbi.nlm.nih.gov/nuccore/AY263491

1. Stijlemans B, Conrath K, Cortez-Retamozo V, Van Xong H, Wyns L, et al. Efficient targeting of conserved cryptic epitopes of infectious agents by single domain antibodies. African trypanosomes as paradigm. J Biol Chem. 2004;279:1256–1261 2. Stijlemans B, Caljon G, Natesan SKA, et al. High Affinity Nanobodies against the Trypanosome brucei VSG Are Potent Trypanolytic Agents that Block Endocytosis. Parsons M, ed. PLoS Pathogens. 2011;7(6):e1002072. doi:10.1371/journal.ppat.1002072. 3.De Vooght L, Caljon G, De Ridder K, Van Den Abbeele J. Delivery of a functional anti-trypanosome Nanobody in different tsetse fly tissues via a bacterial symbiont, Sodalis glossinidius. Microbial Cell Factories. 2014;13:156. doi:10.1186/s12934-014-0156- Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 50