Coding

Part:BBa_K2382012

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 12:47, 29 October 2017 by Herlohuang (Talk | contribs)

EAAAK



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

A rigid linker link two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding.


The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the target protein becomes automatically released from the fusion protein without proteolytic treatment. Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has been successfully employed for many cases of protein purification without the tag of a fusion protein.

Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021

[edit]
Categories
Parameters
None