Coding

Part:BBa_K2271003

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 20:18, 1 November 2017 by Pauline tot (Talk | contribs)


Pex11-Venus with galactose inducible promoter

This part contains the coding sequence for the peroxin Pex11p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None