Composite

Part:BBa_K2407313

Designed by: Xinyu Chen   Group: iGEM17_Tianjin   (2017-10-27)
Revision as of 14:53, 27 October 2017 by 01is01 (Talk | contribs)

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TEF + vox + RFP + Adh1T + Ura3T + vox + β-carotene


Introduction

We use this composite to compare the phenomenon before and after mating. Initially, the Red Fluorescence Protein expresses. When the vika-vox system begin to work, the component between two vox sites will be deleted and the β-carotene gene will synthesize β-carotene. Yeasts turns from red to orange.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2388
    Illegal BglII site found at 4075
    Illegal XhoI site found at 2087
    Illegal XhoI site found at 2219
    Illegal XhoI site found at 3038
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4979
    Illegal BsaI site found at 7334
    Illegal BsaI.rc site found at 176
    Illegal BsaI.rc site found at 1815
    Illegal BsaI.rc site found at 7665
    Illegal SapI site found at 4379


[edit]
Categories
Parameters
None