Composite

Part:BBa_K2305000

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-10-19)
Revision as of 14:21, 27 October 2017 by Buqi (Talk | contribs)


strong promoter, cycle amplification system and GFP/RFP dual fluorescent system

Strong promoter pcat and promoter plux start the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1591
    Illegal BglII site found at 2770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4451
    Illegal AgeI site found at 4563
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64
    Illegal BsaI.rc site found at 3449


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Categories
Parameters
None