Reporter

Part:BBa_K2407012

Designed by: Mingzhe Han   Group: iGEM17_Tianjin   (2017-10-22)
Revision as of 14:31, 27 October 2017 by 01is01 (Talk | contribs)


a modified yEmRFP for use in Yeast from mCherry mRFP

It is the RFP variant sequence that codon-optimized for the expression in Saccharomyces cerevisiae as yeast-enhanced mRFP (yEmRFP) and can combine fluorescence and a purple visible phenotype.

reference

Emiliano A, Monica B, Alessia F, et al. Chemical-Induced Read-Through at Premature Termination Codons Determined by a Rapid Dual-Fluorescence System Based onS.cerevisiae:[J]. Plos One, 2016, 11(4):e0154260.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None