DNA

Part:BBa_K2440036

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 02:05, 31 October 2017 by MINXIAO (Talk | contribs) (→‎Usage and Biology)


miR7514 target sequence

It is the target sequence of miR7514, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker.

Ghr-miR7514 was firstly detected in in cotton ovules.1 MiR7514 is found to be differentially expressed in PHYA1 RNAi cotton and it may be related with the fiber quality of cotton2.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

[edit]
Categories
Parameters
None