DNA

Part:BBa_K2440034:Design

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 12:19, 27 October 2017 by Jhyzxqj (Talk | contribs) (→‎Source)

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miR172 target sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is the reverse complementary sequence of miR172.

Source

We obtained it by annealing of two complementary single-strand oligonucleotides, which were synthesized by company as primers.

References