Composite
Part:BBa_K2455008:Design
Designed by: Jon Fugl Group: iGEM17_UCopenhagen (2017-10-27)
YddG-AroG construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455000)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1847
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 577
Illegal AgeI site found at 639
Illegal AgeI site found at 756 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1154
Design Notes
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Source
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References
Gu, P. et al. (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories, 11(1), p.30.
Wang, J. et al. (2013). Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Applied Microbiology and Biotechnology, 97(17), pp.7587-7596.