![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2275007
Designed by: Cheng-Yen Lin Group: iGEM17_NCKU_Tainan (2017-10-14)
Revision as of 10:29, 27 October 2017 by TedShihncku (Talk | contribs)
PlacI-B0034-NiR cluster
This composite part is composed of R0010, B0034 and K2275001 which encodes nitrite reductase derived from E. coli K-12 MG1655 strain. Then we used Griess reagent assay to test the activity of NiR. The principle and mechanism of Griess reaction are shown below.
When the broth contains nitrite, by adding the Griess reactant which consists of naphthylethylenediamine dihydrochloride, sulphanilamide, phosphoric acid can form a diazonium salt with nitrite. The diazonium salt will have absorbance at wavelength 545 nm. By the reaction, we can further calculate the nitrate conversion.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 286
Illegal BglII site found at 1287
Illegal BglII site found at 2070
Illegal BamHI site found at 567 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2093
Illegal AgeI site found at 551
Illegal AgeI site found at 947
Illegal AgeI site found at 2105 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 401
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Categories
Parameters
None |