Composite

Part:BBa_K2448023

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 18:11, 30 October 2017 by Brumake (Talk | contribs)


Universal Biosensing Chassis (UBC)

Universal Biosensing Chassis (UBC)

Usage and Biology

The Universal Biosensing Chassis (UBC) is the keystone of our project. It aims to provide an answer to the lack of rapid and reliable building methods for transcription-factor based biosensors. Biosensors rely on a basic theoretical principle: a certain concentration of a molecule of interest induces a proportional production of a fluorescent compound. Transcription-factor based biosensors allow the precise and cheap detection or quantification of various chemical compounds.

Using our composite biobrick, one will only need a suitable transcription factor, able to bind to the molecule of interest, and its related promoter. However sometimes, there is no transcription factor in the databases that matches what we are looking for. Here we can use indirect sensing or Sensing-Enabling Metabolic Pathways. Essentially, tools like [http://sensipath.micalis.fr Sensipath] search and design the enzymatic reactions necessary to transform your non-detectable molecule in a detectable one, therefore expanding the repertoire of molecules available to sensing.

Apart from its fast building design, the UBC has been engineered to be used in high throughput procedures such as screening. Indeed, we originally designed it for a new enzyme engineering process for D-psicose production, using a psicose biosensor to identify the enzyme mutants showing the best activity. In addition, by its design, the UBC allows fast cloning: depending on what is to be inserted into the UBC and one’s mastery of the Golden Gate Assembly, a functional biosensor can be obtained in less than a week.

For more information: http://2017.igem.org/Team:Evry_Paris-Saclay


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1033
    Illegal NheI site found at 1056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1005
    Illegal XhoI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 128
    Illegal AgeI site found at 251
    Illegal AgeI site found at 2154
    Illegal AgeI site found at 2266
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None